An EcR homolog from the filarial parasite, Dirofilaria immitis requires a ligand-activated partner for transactivation.
Identifieur interne : 005A34 ( Main/Exploration ); précédent : 005A33; suivant : 005A35An EcR homolog from the filarial parasite, Dirofilaria immitis requires a ligand-activated partner for transactivation.
Auteurs : Cathy Shea [États-Unis] ; Jennifer Richer ; George Tzertzinis ; Claude V. MainaSource :
- Molecular and biochemical parasitology [ 1872-9428 ] ; 2010.
Descripteurs français
- KwdFr :
- ADN des helminthes (), ADN des helminthes (génétique), Activation de la transcription, Alignement de séquences, Analyse de séquence d'ADN, Animaux, Cellules NIH 3T3, Dirofilaria immitis (génétique), Dirofilaria immitis (physiologie), Données de séquences moléculaires, Gènes rapporteurs, Humains, Liaison aux protéines, Ligands, Récepteurs X des rétinoïdes (métabolisme), Récepteurs aux stéroïdes (génétique), Récepteurs aux stéroïdes (métabolisme), Régulation de l'expression des gènes, Souris, Séquence d'acides aminés, Test de retard de migration électrophorétique.
- MESH :
- génétique : ADN des helminthes, Dirofilaria immitis, Récepteurs aux stéroïdes.
- métabolisme : Récepteurs X des rétinoïdes, Récepteurs aux stéroïdes.
- physiologie : Dirofilaria immitis.
- ADN des helminthes, Activation de la transcription, Alignement de séquences, Analyse de séquence d'ADN, Animaux, Cellules NIH 3T3, Données de séquences moléculaires, Gènes rapporteurs, Humains, Liaison aux protéines, Ligands, Régulation de l'expression des gènes, Souris, Séquence d'acides aminés, Test de retard de migration électrophorétique.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, DNA, Helminth (chemistry), DNA, Helminth (genetics), Dirofilaria immitis (genetics), Dirofilaria immitis (physiology), Electrophoretic Mobility Shift Assay, Gene Expression Regulation, Genes, Reporter, Humans, Ligands, Mice, Molecular Sequence Data, NIH 3T3 Cells, Protein Binding, Receptors, Steroid (genetics), Receptors, Steroid (metabolism), Retinoid X Receptors (metabolism), Sequence Alignment, Sequence Analysis, DNA, Transcriptional Activation.
- MESH :
- chemical , chemistry : DNA, Helminth.
- chemical , genetics : DNA, Helminth, Receptors, Steroid.
- genetics : Dirofilaria immitis.
- chemical , metabolism : Receptors, Steroid, Retinoid X Receptors.
- physiology : Dirofilaria immitis.
- Amino Acid Sequence, Animals, Electrophoretic Mobility Shift Assay, Gene Expression Regulation, Genes, Reporter, Humans, Ligands, Mice, Molecular Sequence Data, NIH 3T3 Cells, Protein Binding, Sequence Alignment, Sequence Analysis, DNA, Transcriptional Activation.
Abstract
Filarial parasites are responsible for several serious human diseases with symptoms such as lymphoedema, elephantiasis, and blindness. An understanding of how these parasites pass through developmental checkpoints may suggest potential targets for intervention. A useful model system for the study of the human parasites is the closely related nematode, D. immitis, the causative agent of dog heartworm disease. Ecdysteroids have been identified in filarial nematodes and have been shown to have a biological affect both on molting and microfilarial production. The ecdysteroid, 20-hydroxyecdysone and its receptor, EcR, have a well-characterized developmental role in insects, where it is involved in the control of molting and metamorphosis. We have identified a D. immitis nuclear receptor, DiEcR that shows strong sequence similarity to the insect EcR and shares many of its biochemical properties, including ligand-dependent activation of transcription. However, unlike most insect EcRs, DiEcR requires a ligand-activated RXR partner to exhibit ligand-dependent transcriptional activation of a reporter gene in tissue culture.
DOI: 10.1016/j.molbiopara.2010.02.002
PubMed: 20170689
Affiliations:
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Le document en format XML
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Maina</name></author></analytic><series><title level="j">Molecular and biochemical parasitology</title><idno type="eISSN">1872-9428</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>DNA, Helminth (chemistry)</term><term>DNA, Helminth (genetics)</term><term>Dirofilaria immitis (genetics)</term><term>Dirofilaria immitis (physiology)</term><term>Electrophoretic Mobility Shift Assay</term><term>Gene Expression Regulation</term><term>Genes, Reporter</term><term>Humans</term><term>Ligands</term><term>Mice</term><term>Molecular Sequence Data</term><term>NIH 3T3 Cells</term><term>Protein Binding</term><term>Receptors, Steroid (genetics)</term><term>Receptors, Steroid (metabolism)</term><term>Retinoid X Receptors (metabolism)</term><term>Sequence Alignment</term><term>Sequence Analysis, DNA</term><term>Transcriptional Activation</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN des helminthes ()</term><term>ADN des helminthes (génétique)</term><term>Activation de la transcription</term><term>Alignement de séquences</term><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Cellules NIH 3T3</term><term>Dirofilaria immitis (génétique)</term><term>Dirofilaria immitis (physiologie)</term><term>Données de séquences moléculaires</term><term>Gènes rapporteurs</term><term>Humains</term><term>Liaison aux protéines</term><term>Ligands</term><term>Récepteurs X des rétinoïdes (métabolisme)</term><term>Récepteurs aux stéroïdes (génétique)</term><term>Récepteurs aux stéroïdes (métabolisme)</term><term>Régulation de l'expression des gènes</term><term>Souris</term><term>Séquence d'acides aminés</term><term>Test de retard de migration électrophorétique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Helminth</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Helminth</term><term>Receptors, Steroid</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Dirofilaria immitis</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN des helminthes</term><term>Dirofilaria immitis</term><term>Récepteurs aux stéroïdes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Receptors, Steroid</term><term>Retinoid X Receptors</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Récepteurs X des rétinoïdes</term><term>Récepteurs aux stéroïdes</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Dirofilaria immitis</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Dirofilaria immitis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Electrophoretic Mobility Shift Assay</term><term>Gene Expression Regulation</term><term>Genes, Reporter</term><term>Humans</term><term>Ligands</term><term>Mice</term><term>Molecular Sequence Data</term><term>NIH 3T3 Cells</term><term>Protein Binding</term><term>Sequence Alignment</term><term>Sequence Analysis, DNA</term><term>Transcriptional Activation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN des helminthes</term><term>Activation de la transcription</term><term>Alignement de séquences</term><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Cellules NIH 3T3</term><term>Données de séquences moléculaires</term><term>Gènes rapporteurs</term><term>Humains</term><term>Liaison aux protéines</term><term>Ligands</term><term>Régulation de l'expression des gènes</term><term>Souris</term><term>Séquence d'acides aminés</term><term>Test de retard de migration électrophorétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Filarial parasites are responsible for several serious human diseases with symptoms such as lymphoedema, elephantiasis, and blindness. An understanding of how these parasites pass through developmental checkpoints may suggest potential targets for intervention. A useful model system for the study of the human parasites is the closely related nematode, D. immitis, the causative agent of dog heartworm disease. Ecdysteroids have been identified in filarial nematodes and have been shown to have a biological affect both on molting and microfilarial production. The ecdysteroid, 20-hydroxyecdysone and its receptor, EcR, have a well-characterized developmental role in insects, where it is involved in the control of molting and metamorphosis. We have identified a D. immitis nuclear receptor, DiEcR that shows strong sequence similarity to the insect EcR and shares many of its biochemical properties, including ligand-dependent activation of transcription. However, unlike most insect EcRs, DiEcR requires a ligand-activated RXR partner to exhibit ligand-dependent transcriptional activation of a reporter gene in tissue culture.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Massachusetts</li></region></list><tree><noCountry><name sortKey="Maina, Claude V" sort="Maina, Claude V" uniqKey="Maina C" first="Claude V" last="Maina">Claude V. Maina</name><name sortKey="Richer, Jennifer" sort="Richer, Jennifer" uniqKey="Richer J" first="Jennifer" last="Richer">Jennifer Richer</name><name sortKey="Tzertzinis, George" sort="Tzertzinis, George" uniqKey="Tzertzinis G" first="George" last="Tzertzinis">George Tzertzinis</name></noCountry><country name="États-Unis"><region name="Massachusetts"><name sortKey="Shea, Cathy" sort="Shea, Cathy" uniqKey="Shea C" first="Cathy" last="Shea">Cathy Shea</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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